Click instrument to see more details. For detailed information on objective and fluorescence filter properties, see these specification sheets. For videos and other resources about how to use the microscopes, see this practical resources page.
Microscopes
- Leica SP8 Stellaris Laser Scanning Confocal with Lightning and Falcon (inverted)
- Nikon Eclipse Ti-2 (inverted) (color/DIC/fluorescence/high-content-acquisition)
- Nikon TE2000 C2si Laser Scanning Confocal and Widefield Microscope (inverted)
- Leica DM6b (color/DIC/polarization/fluorescence)
- Zeiss Axioscope.A1 (color/fluorescence)
- Zeiss Discovery V12 Stereoscope
- Zeiss SV6 Stemi Stereoscope
- Leica Macroscope
WideTEK25 Large-Format Flat-Bed Scanner
Leica SP8 Stellaris Laser Scanning Confocal
The aquisition of this microscope system was funded by NSF MRI grant DBI-2214633.
- Technical Specifications
- Inverted microscope base
- DIC/fluorescence
- Objectives: 10x, 20x, 20x (multi-immersion), 40x (glycerol), 100x (oil)
- Lightning enhanced resolution
- Falcon Fluorescence Lifetime Imaging (FLIM)
- Tokai-Hit incubation chamber (temperature, CO2, humidity)
- Focus stabilization
- Peizo-z-drive
Nikon Eclipse Ti-2
The acquisition of this microscope system was funded by NSF MRI grant DBI-1725885.
- Technical Specifications
- Inverted microscope base
- DIC/fluorescence (phase contrast with 10x only)
- Objectives: 4x, 10x, 20x, 40x (1.3NA), 60x (1.4NA), and 100x (1.45NA)
- Fully motorized and encoded
- Perfect-focus
- Tokai-Hit incubation chamber (temperature, CO2, humidity)
- SPECTRA-X LED fluorescence light source
- Fluorescence filters: blue, cyan, green, yellow, orange, red, far-red, + multi-pass fluorescence filters for rapid acquisition
- Hamamatsu ORCA-Flash 4.0 V3 sCMOS monochrome camera
- DS-RI2 colorc cMOS camera
- High-content acquisition automation capabilities
Nikon TE2000 C2si Laser Scanning Confocal and Widefield Microscope
The acquisition of this microscope system was funded by NSF MRI grant DBI-0619207.
- Technical Specifications
- Inverted microscope base
- DIC/Fluorescence
- Objectives: 4x, 10x, 20x, 20x (extra-long working distance), 40x, 60x (1.4NA), and 100x (1.4NA)
- Motorized focus with focus-drift correction
- Motorized stage
- Active vibration isolation table
- Widefield mode
- Fluorescence filters: blue, green, orange, red, far-red
- Hamamatsu ORCA-Flash 4.0 V3 sCMOS monochrome camera
- Nikon DSFi1 color camera
- Confocal mode
- Lasers: 405, 457, 488, 515, 561, and 647 nm
- Three-channel (plus transmitted) detection
- Spectral detector
Leica DM6b
- Technical Specifications
- DIC/Fluorescence
- Objectives: 5x, 10x, 20x, 40x, 100x
- Fluorescence filters: blue, green, red
- Leica DFC 7000T color CCD camera
- LAS X imaging software
Zeiss Axioscope.A1
- Technical Specifications
- Brightfield/Fluorescence
- Objectives: 5x, 10x, 20x, 40x, 63x (NA1.25)
- Fluorescence filters: blue, green, red
- QImaging PV1 color CCD camera
- Ocular imaging software
- BioQuant imaging and analysis package
Zeiss Discovery V12 Stereoscope
- Objective: Achromat S 1.0x
- Axiocam 305 color camera
- Diascopic and incident light illumination
- Oblique and darkfield illumination
- Polarization
Zeiss SV6 Stemi Stereoscope
- Objectives: 0.63x, 1.0x
- Insight 2 color camera
- SPOT imaging software
Leica Macroscope
- Objectives: 0.32-2x
- Motorized focus drive for capturing “z-stacks”
Image Analysis Workstations
- HP computer with Nikon Elements, including deconvolution and high-content analysis packages
- Dell Optiplex computer with Nikon Elements, Adobe Photoshop, Adobe Illustrator, and FIJI/ImageJ
WideTEK25 Large-Format Flat-bed Scanner
- 25x18.5 inch scan area
- Optional back-light