Dr. Kubow will assign specific sections or items based on the type(s) of microscopy that you want to do. Complete only the sections that have been assigned to you. Ask Dr. Kubow if you are unsure.

Videos with a * are from the Microcourses YouTube channel, produced by Dr. Jennifer Waters at Harvard. All other videos were produced by Dr. Kubow  and may require you to sign-in using your JMU creditials.

1. The Basics (do this first!)
   1. Read: Microscopy Facility User Policy
   2. Complete: User Registration Form (print, sign, and bring to your first training)
   3. Read: Resolution Overview
   4. Read: Digital Imaging Overview
2. Brightfield ("normal") microscopy
   1. Read: Brightfield Microscopy Overview
3. Phase Contrast Microscopy
   1. Complete Tutorial #2 (Brightfield Microscopy) first.
   2. Read: Phase Contrast Microscopy Overview
4. DIC microscopy
   1. Complete Tutorial #2 (Brightfield Microscopy) first.
   2. Read: DIC Microscopy Overview
5. Fluorescence microscopy
   1. Read: Fluorescence Microscopy Overview
   2. Watch: *Choosing a Filter Set
   3. If you will be using more than one fluorescent probe, watch: *Imaging Multiple Fluorophores (up to 8:40)
6. Confocal microscopy
   
   1. Complete Tutorial #5 (fluorescence microscopy) first.
   2. Read: Confocal Microscopy Overview
   3. Watch: *The Confocal Principle
7. Advanced Techniques
8. 1. If you will be using Lightning mode for enhanced resolution, read the Lightning Overview
   2. If you will be performing Fluorescence Lifetime Imaging (FLIM), read the FLIM Overview
9. Data Management
   1. Read: Microscopy Data Management for Students
   2. Complete the Data Management Plan Worksheet (docx or pdf) and bring to your next training.