Associate Professor, Biophysical Chemistry
Year Started at JMU: 2014
Contact Info

Research Description

Most of our work is focused on biochemical and biophysical studies of cytochromes, a large class of heme-containing proteins involved in energy transduction and biological catalysis. We use a wide range of experimental and computational techniques including: DNA cloning and mutagenesis, recombinant protein expression and isolation, time-resolved absorbance and fluorescence spectroscopy, mass spectroscopy, isothermal calorimetry, small- and wide-angle X-ray scattering, and molecular dynamics simulations.

Related Scholarship
  • Charge and energy transfer in proteins
  • Small- and wide-angle X-ray scattering
  • Time-resolved laser spectroscopy
  • PhD in Biophysics and Computational Biology, 2010, University of Illinois at Urbana-Champaign
Select Publications
  • Kokhan, O., Marzolf, D.R. (2019) Detection and quantification of transition metal leaching in metal affinity chromatography with hydroxynaphthol blue. Anal. Biochem., 528, 113347.
  • Kokhan, O., Ponomarenko, N., Pokkuluri, R.P., Schiffer, M., Mulfort, K.L., Tiede, D.M. (2015) Bidirecetional photoinduced electron transfer in Ruthenium(II)-tris-bipyridyl modified PpcA, a multi-heme c-type cytochrome from Geobacter sulfurreducens. J. Phys. Chem. B, 119, 7612-7624.
  • Kokhan, O., Ponomarenko, N., Pokkuluri, P.R., Schiffer, M., Tiede, D.M. (2014) Multimerization of solution-state proteins by tetrakis(4-sulfonatophenyl) porphyrin, Biochemistry, 53, 5070-5079.

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