Click instrument to see more details. For detailed information on objective and fluorescence filter properties, see these specification sheets. For videos and other resources about how to use the microscopes, see this practical resources page.

Microscopes

Image Analysis Workstations

WideTEK25 Large-Format Flat-Bed Scanner

Leica SP8 Stellaris Laser Scanning Confocal

The aquisition of this microscope system was funded by NSF MRI grant DBI-2214633.

  • Technical Specifications
  • Inverted microscope base
  • DIC/fluorescence
  • Objectives: 10x, 20x, 20x (multi-immersion), 40x (glycerol), 100x (oil)
  • Lightning enhanced resolution
  • Falcon Fluorescence Lifetime Imaging (FLIM)
  • Tokai-Hit incubation chamber (temperature, CO2, humidity)
  • Focus stabilization
  • Peizo-z-drive

Nikon Eclipse Ti-2

The acquisition of this microscope system was funded by NSF MRI grant DBI-1725885.

  • Technical Specifications
  • Inverted microscope base
  • DIC/fluorescence (phase contrast with 10x only)
  • Objectives: 4x, 10x, 20x, 40x (1.3NA), 60x (1.4NA), and 100x (1.45NA)
  • Fully motorized and encoded
  • Perfect-focus
  • Tokai-Hit incubation chamber (temperature, CO2, humidity)
  • SPECTRA-X LED fluorescence light source
  • Fluorescence filters: blue, cyan, green, yellow, orange, red, far-red, + multi-pass fluorescence filters for rapid acquisition
  • Hamamatsu ORCA-Flash 4.0 V3 sCMOS monochrome camera
  • DS-RI2 colorc cMOS camera
  • High-content acquisition automation capabilities

Nikon TE2000 C2si Laser Scanning Confocal and Widefield Microscope

The acquisition of this microscope system was funded by NSF MRI grant DBI-0619207.

  • Technical Specifications
  • Inverted microscope base
  • DIC/Fluorescence
  • Objectives: 4x, 10x, 20x, 20x (extra-long working distance), 40x, 60x (1.4NA), and 100x (1.4NA)
  • Motorized focus with focus-drift correction
  • Motorized stage
  • Active vibration isolation table
  • Widefield mode
    • Fluorescence filters: blue, green, orange, red, far-red
    • Hamamatsu ORCA-Flash 4.0 V3 sCMOS monochrome camera
    • Nikon DSFi1 color camera
  • Confocal mode
    • Lasers: 405, 457, 488, 515, 561, and 647 nm
    • Three-channel (plus transmitted) detection
    • Spectral detector

Leica DM6b

  • Technical Specifications
  • DIC/Fluorescence
  • Objectives: 5x, 10x, 20x, 40x, 100x
  • Fluorescence filters: blue, green, red
  • Leica DFC 7000T color CCD camera
  • LAS X imaging software

Zeiss Axioscope.A1

  • Technical Specifications
  • Brightfield/Fluorescence
  • Objectives: 5x, 10x, 20x, 40x, 63x (NA1.25)
  • Fluorescence filters: blue, green, red
  • QImaging PV1 color CCD camera
  • Ocular imaging software
  • BioQuant imaging and analysis package

Zeiss Discovery V12 Stereoscope

  • Objective: Achromat S 1.0x
  • Axiocam 305 color camera
  • Diascopic and incident light illumination
  • Oblique and darkfield illumination
  • Polarization

Zeiss SV6 Stemi Stereoscope

  • Objectives: 0.63x, 1.0x
  • Insight 2 color camera
  • SPOT imaging software

Leica Macroscope

  • Objectives: 0.32-2x
  • Motorized focus drive for capturing “z-stacks”

Image Analysis Workstations

  • HP computer with Nikon Elements, including deconvolution and high-content analysis packages
  • Dell Optiplex computer with Nikon Elements, Adobe Photoshop, Adobe Illustrator, and FIJI/ImageJ

WideTEK25 Large-Format Flat-bed Scanner

  • 25x18.5 inch scan area
  • Optional back-light

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Biology Department: Light Microscopy and Imaging Facility
Bioscience Building
MSC 7801
951 Carrier Drive
Room 2033 and 2035
Harrisonburg, Virginia 22807