Professor, ISAT, IA

raabrw@jmu.edu

Contact Info

Education
  • Post Doctoral – DNAX Research Inst.
  • Ph.D. Texas A&M University
  • M.S.  Texas A&M University
  • B.A.  University of California, Santa Barbara
Experience

Dr. Raab teaches courses in biotechnology and awareness and understanding of WMDs. He serves as a regional HazMat responder, and educator for the  Fire Departments of Harrisonburg, and Rockingham County in Virginia.  Provides  instruction and training for National Guard Civil Support Teams. In addition,  also serves as a volunteer firefighter.  He joined the JMU faculty in Fall 2001. He received a B.A. in Biochemistry and Molecular Biology from University of California, Santa Barbara. He received an M.S. degree in Organic Chemistry and a Ph.D. in Molecular Biology and Genetics from Texas A&M University. Prior to joining JMU, he spent 15 years in the biotech industry as a research scientist at IDEC Pharmaceuticals, and Affymax Research Institute. He has taught Molecular Biology and Genetics at the University of California, Berkeley. His research interests are the cloning, expression, and purification of genes from pathogenic bacteria, and viruses for use as potential vaccines, therapeutics, and/or for diagnostic reagents for the U.S. Army Medical Research Institute of Infectious Diseases. Currently working on cloning various forms of the human eye protein lacritin for therapeutical of dye-eye syndrome and bacterial eye infections. 

Certified Instructor
  • WMD Radiological/Nuclear Awareness - National Security Technologies
  • WMD Standardized Awareness Trainer - Center for Domestic Preparedness
  • Fundamentals Course for Radiological Response - Center for Domestic Preparedness
  • Radiological Training for Hospital Personnel - Center for Domestic Preparedness
  • Avanced Chem/Bio Integrated Response - Joint Operational Testing and Training
  • Hospital Emergency Response Teams - Center for Domestic Preparedness
  • Integrated Command Systems 300 and 400 – Virginia Dept. of Emergency Management
Publications
  • Robert L. McKown, Erin V. Coleman Frazier, Kaneil K. Zadrozny, Andrea M. Deault, Ronald W. Raab Denise S. Ryan, Rose K. Sia, Jae K. Lee, and Grodon W. Laurie.  A Cleavage-potentiated Fragment of Tear Lacritin Is Bactericidal.  The Journal of Biological Chemistry  Vol. 289, No. 32 pp. 22172, August 8, 2014

  • Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler MF 4th, Lee JK, Laurie GW.  Lacritin rescues stressed epithelia via rapid forkhead box O3 (FOXO3)-associated autophagy that restores metabolism.  J Biol Chem. 2013 Jun 21;288(25):18146-61. PMID: 23640897

  • Zhang Y, Wang N, Raab RW, McKown RL, Irwin JA, Kwon I, van Kuppevelt TH, Laurie GW.Targeting of heparanase-modified syndecan-1 by prosecretory mitogen lacritin requires conserved core GAGAL plus heparan and chondroitin sulfate as a novel hybrid binding site that enhances selectivity.  J Biol Chem. 2013 Apr 26;288(17):12090-101. PMID:23504321

  • McKown RL, Raab RW, Kachelries P, Caldwell S, Laurie GW.  Conserved regional 3' grouping of rare codons in the coding sequence of ocular prosecretory mitogen lacritin.  Invest Ophthalmol Vis Sci. 2013 Mar 19;54(3):1979-87

  • Yinghui Zhang, Ningning Wang, Ronald W. Raab, Robert L. McKown, Jacob A. Irwin, Inchan Kwon, Toin H. van Kuppevelts, and GordonW. Laurie.  Targeting of Heparanase-Modified Syndecan-1 by Prosecretory Mitogen Lacritin Requires Conserved Core ‘GAGAL’ Plus Heparan and Chondroitin Sulfate as a Novel Hybrid Binding Site that Enhances Selectivity.  2013 J. Biol. Chem. March 15 issue

  • Velez V FRomano JAMcKown RLGreen KZhang LRaab RWRyan DSHutnik CMFrierson HF JrLaurie GW.  Tissue transglutaminase is a negative regulator of monomeric lacritin bioactivity.  Invest Ophthalmol Vis Sci. 2013 Mar 1;54(3):2123-32

  • Michelle M. Giffin, Ronald W. Raab, Melissa Morganstern, and Charles D. Sohaskey.  Mutational Analysis of the Respiratory Nitrate Transporter NarK2 of Mycobacterium tuberculosis PloS One.  2012; 7(9): e45459

  • Michelle M. Giffin, Lucia Modesti, Ronald W. Raab, Lawrence G. Wayne, and Charles D. Sohaskey. ald of Mycobacterium tuberculosis Encodes both the Alanine Dehydrogenase and the Putative Glycine Dehydrogenase  Journal of Bacteriology.  2012, 194(5): 1045-1054

  • Zhang YMcKown RLRaab RWRapraeger ACLaurie GW.  Focus on molecules: syndecan-1.  Exp Eye Res. (2011) ;93(4):329-30.  PMID:  20599970

  • Bradford S Powell, Ph.D.; Jeff T Enama; Ronald W Raab, Ph.D.; Robert  L McKown, Ph.D.; George Coffman, Ph.D.; Gerard P Andrews, Ph.D. Purification and characterization of a recombinant Yersinia pestis V-F1 "Reversed" fusion protein for use as a new subunit vaccine against plague Protein Expression and Purification, 2011, 76, 136-144

  • Jenkins, Tiffany, Smith, Josh, Zimmerman, Benjamin, and Raab, Ronald.  Oxygen Depletion in a Level A Hazmat Suit.  Fire Engineering.  2010 November vol.163 #11

  • Robert L. McKown, Ningning Wang, Ronald W. Raab, Roy Karnati, Yinghui Zhang, Patricia

  • B. Williams, Gordon W. Laurie (2009) Lacritin and other new proteins of the lacrimal functional unit.  Experimental Eye Research.  2009 May; 88(5): 848-58

  • Peisong Ma, Ningning Wang, Rober L. McKown, Ronald W. Raab, and Gordon W. Laurie.  Focus on Molecules: Lacritin.  Experimental Eye research (2008) 86

  • Ronald Raab, and Wieslaw Swietnicki.  Yersinia pestis YopD15-0287 fragment is partially unfolded in the native state.  Protein Expression and Purification (2008) 58 

  • George L. Coffman, Ronald W. Raab, and Robert L. McKown.  Expression of Ebola Virus Proteins in a Eucaryotic Cell System for Production of Critical Diagnostics to Address the Threat of Bioterrorism.  George Mason University Press. 2007

  • Peisong Ma, Ningning Wang, Robert L. McKown, Ronald W. Raab, and Gordon W. Laurie.  Focus on Molecules: Lacritin.  Experimental Eye Research. 2007

  • Peisong Ma, Shannon L. Beck, Ronald W. Raab, Robert L. McKown, George L. Coffman, William J. Chirico, Alan C. Rapraeger, and Gordon L. Laurie.  Deglycosylation of Co-recetptor Syndecan-1 is Required for Core Protein Binding of Epithelial Restricted Prosecretory Mitogen Lacritin.   The Journal of Cell Biology (2006) Vol. 174. No. 7

  • W.G. Tucker, D. J. Lawrence, T.C. DeVore, R.W. Raab, and G.L. Coffman.  An IAQ Sensing and Alarm System for Chemical and Biological Substances.  Proceedings of the Healthy Buildings 2006 Conference.  June 2006

  • Jiahu Wang, Staci C. Walton, Ningning Wang, Ronald W. Raab, Robert L. McKown, Shannon L. Beck, George L. Coffman, Isa Hussaini, and Gordon W. Laurie.  Restricted Epithelial Proliferation by Lacritin/Inactive PKC/NFATC2 Pathway.  The Journal of Cell Biology (2006) Vol. 174. No. 5, 689-700

  • Goodin JL, RW Raab, RL McKown, GL Coffman, BS Powell, JT Enama, JA Ligon, GP Andrews. Yersinia pestis outer membrane type III secretion protein YscC: expression, purification, characterization, and induction of specific antiserum. Protein Expr Purif. 2005

  • Peisong Ma, Shannon Beck, Ron Raab, Robert McKown, George Coffman, Gordon W. Laurie.  Identification of Syndecan-1 as a Coreceptor for Prosecretory Mitogen Lacritin.  The Ocular Surface.  Vol.3. No1.  January 2005 

  • J. L. Goodin, R. W. Raab, R. L. McKown, G. Coffman, B. S. Powell, J. T. Enama, J. A. Ligon, and G. P. Andrews.  Yersinia pestis outer membrane type III secretion protein YscC:  expression, purification, characterization, and induction of specific antiserum. (2005) Protein Expr. & Purif. 40, 152-163

  • W. Gene Tucker, David Lawrence, Thomas DeVore, and Ronald Raab.  Development of an Indoor Air Quality Sensing and Alert/Alarm System.  (2004) Critical Infrastructure Protection Program, Workshop II Working Papers.  George Mason University Press. Pg. 187

  • Ronald Raab, Robert McKown, and George Coffman.  Development of Recombinant Biological Vaccines and Critical Diagnostics to Address the Threat of Bioterrorism. (2004) Critical Infrastructure Protection Program, Workshop II Working Papers.  George Mason University Press. Pg. 199

  • Ronald Raab, Robert Mckown, and George Coffman.  The Use of E. Coli Expressed Proteins    of Yersina Pestis for the Development of Vaccines Against Pheumonic Plague. (2004) Virginia J. Science. Vol. 55. (No. 1&2) 14

  • R.L. McKown, S.J. Aherne, L. Stehling, R. W. Raab, G. L. Coffman, and G.W. Laurie.  Mutational Analysis of Recombinant Lacritin, A Novel Secretion Enhancing Factor From the Human Lacrimal Gland. (2004) Virginia J. Science. Vol. 55. (No. 1&2) 19-20

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