A-to-Z Index


Christine A Hughey, Ph.D.

Associate Professor
Department of Chemistry and Biochemistry
Group Website

Education, Honors, Awards

  • Received 1st place award from the Undergraduate Analytical Research Program, Society for Analytical Chemists of Pittsburgh (2009)
  • Achievement Award for Excellence in Teaching, Chapman University (2005)
  • Associate Professor, James Madison University (2010-present)
  • Associate Professor, Chapman University, Orange CA (2009-2010)
  • Assistant Professor, Chapman University, Orange CA (2002-2009)
  • Ph.D. Analytical Chemistry, Florida State University, Tallahassee FL (2002)
  • B.S. Chemistry, Berry College, Rome GA (1998)

Research Interests

Ionization and characterization of acidic compounds by negative ion electrospray (ESI) mass spectrometry with emphasis on:
  • Effect of solvent and solution conditions on negative ion ESI response
  • Prediction of negative ion ESI response
  • Characterization of acidic compounds in complex environmental and agricultural samples

Research Description

Dr. Christine A. Hughey is an analytical chemist whose research centers on method development for the characterization, quantification and study of acidic polar compounds in complex environmental and agricultural samples by negative ion electrospray ionization mass spectrometry (ESI MS).   The interdisciplinary nature of her research affords her the opportunity to work on a variety of projects from the characterization of flavonoids in antioxidant-rich food extracts to field studies that investigate the use of naphthenic acids as molecular indicators of in-situ bioremediation in crude oil-contaminated soil.  These applied projects have led her to pursue studies that investigate the fundamental mechanisms involved in negative ion ESI, which are not well understood.  Specifically, she is interested how solvent and solution conditions effect the ionization of acidic compounds.  Improved understanding of ESI ionization mechanisms will lead to development of a predictive model of negative ion ESI response.

Selected Recent Publications

  • Huffman, B.A.; Poltash, M.L.; Hughey, C.A. "Effect of polar protic and polar aprotic solvents on negative ion electrospray ionization and chromatographic separation of small acidic molecules."  Submitted to Analytical Chemistry, August 2012.
  • Barrett, A.; Hughey, C.A.; Straut, C.; Howell, A.; Ndou, T.; Dai, Z.; Kaletune, G. "Inhibition of α-amylase and glucoamylase by proanthocyanidins and condensed tannins isolated from grape, pomegranate, cranberry and cocoa." Submitted to Journal of Agriculture and Food Chemistry, June 2012.
  • Hughey, C.A.; Janusziewicz, R.; Minardi, C.S.; Phung, J.; Huffman, B.A.; Reyes, L.; Wilcox, B.E.; Prakash, A. "Distribution of almond polyphenols in blanch water and skins as a function of blanching time and temperature.",  Food Chemistry, 2012, 131, 1165-1173.  DOI: 10.1016/j.foodchem.2011.09.093
  • Teets, A.S.; Minardi, C.S.; Sundararaman, M.; Hughey, C.A.; Were, L.M.  “Extraction, identification and quantification of flavonoids in electron beam irradiated almond skin powder,” Journal of Food Science, 2009, 74, C298-305, DOI: 10.1111/j.1750-3841.2009.01112.x
  • Hughey, C. A.; Minardi, C. S.;  Galasso-Roth, S. A.; Paspalof, G. B.; Mapolelo, M. M.; Rodgers, R. P.; Marshall, A. G.; Ruderman, D. L. “Naphthenic acids as indicators of crude oil biodegradation in soil, based on semi-quantitative ESI FT-ICR MS,” Rapid Communications in Mass Spectrometry, 2008, 22, 3968-3976. DOI: 10.1002/rcm.3813
  • Hughey, C.A.; Wilcox, B.E.; Minardi, C. S.; Takehara, C. W.; Sundararaman, M.; Were, L. M. “Capillary liquid chromatography mass spectrometry for the rapid identification and quantification of almond flavonoids,” Journal of Chromatography A, 2008, 119, 259-265. DOI: 10.1016/j.chroma.2008.03.079
  • Prasetyo, M., Chia, M., Hughey, C. A., Were, L. M.  “Utilization of electron beam irradiated almond skin powder as a natural antioxidant in ground top round beef,” Journal of Food Science, 2008, 73, T1-T6. DOI: 10.1111/j.1750-3841.2007.00553.x
  • Hughey, C. A.; Galasso, S. A.; Zumberge, J. “Detailed compositional comparison of acidic NSO compounds in biodegraded reservoir and surface crude oils by negative ion electrospray Fourier transform ion cyclotron resonance mass spectrometry,” Fuel, 2007, 86, 758-769. DOI: 10.1016/j.fuel.2006.08.029
  • Hughey, C. A.; Rodgers, R. P.; Marshall, A. G.; Walters, C. C.; Qian, K.; Mankiewicz, P. “Acidic and neutral polar NSO compounds in Smackover oils of different thermal maturity revealed by electrospray high field Fourier transform ion cyclotron resonance mass spectrometry." Organic Geochemistry, 2004, 35, 863-880. DOI: 10.1016/j.orggeochem.2004.02.008
  • Hughey, C. A.; Rodgers, R. P.; Marshall, A. G. “Resolution of 11,000 chemically distinct components in a single electrospray ionization Fourier transform ion cyclotron resonance mass spectrum of crude oil,” Analytical Chemistry, 2002, 74, 4145-4149. DOI: 10.1021/ac020146b.
  • Qian, K.; Robbins, W. K.; Hughey, C. A.; Cooper, H. J.; Rodgers, R. P.; Marshall, A. G. “Resolution and identification of 3000 crude acids in heavy petroleum by negative-ion microelectrospray high-field Fourier transform ion cyclotron resonance mass spectrometry,” Energy and Fuels, 2001, 15, 1505-1511. DOI: 10.1021/ef010111z
  • Hughey, C. A.; Hendrickson, C. L.; Rodgers, R. P.; Marshall, A. G.; Qian, K. "Kendrick mass defect spectrum: A compact visual analysis for ultrahigh-resolution broadband mass spectra," Analytical Chemistry, 2001, 73, 4676-4681. DOI: 10.1021/ac010560w