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CISAT Safety Plan - Chemical Safety

Combustibles and Flammables:

Flammable liquids should not be heated with an open flame, hot plate or uninsulated resistance heaters. A heating mantle, steam bath or hot water bath should be used.

Flammable chemicals should not be stored near any source of ignition, spark or open flame. When transferring solvents from one container to another, if the potential for sparking exists, the containers should be electrically "grounded."

Since long-term (chronic) exposures to solvents can cause damage to specific organs, as the kidneys and the liver, and since many halogenaged solvents are carcinogens and/or teratogens,

  • solvents are to be handled in an exhaust hood or a well-ventilated area, and
  • large quantities of flammable reagents should be stored in appropriate safety cabinets.

First-aid:

  • Remove any clothing saturated with spilled solvent
  • Wash affected skin areas with soap and water
  • Flush eyes with water at an eyewash station for at least 15 minutes
  • For severe exposures due to inhalation, remove victim to fresh air, keep warm and still, seek medical attention
  • For ingestion, keep victim warm and seek medical attention
  • IN ALL CASES, CHECK THE MSDS FOR THE CHEMICAL (IF KNOWN) FOR MORE SPECIFIC FIRST-AID INFORMATION

Special notes concerning combustibles and flammables used in ISAT laboratories:

Butyl alcohol, normal

  • COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • STORAGE: IN DARK BOTTLE ONLY.
  • SPILLAGE/DISPOSAL:
    • SHUT OFF ALL POSSIBLE SOURCES OF IGNITION.
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES.
    • MOP UP WITH PLENTY OF WATER.
    • VENTILATE AREA WELL.

Chloroform

  • NONCOMBUSTIBLE, BUT KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES TO PREVENT FORMATION OF TOXIC GAS.
  • CARCINOGEN
  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • STORAGE: IN DARK BOTTLE ONLY.
  • SPILLAGE/DISPOSAL:
    • SHUT OFF ALL POSSIBLE SOURCES OF IGNITION
    • WEAR BREATHING APPARATUS AND GLOVES
    • PERSONS NOT INVOLVED IN CLEAN-UP SHOULD BE INSTRUCTED TO KEEP A SAFE DISTANCE.
    • APPLY DISPERSING AGENT IF AVAILABLE AND WORK TO AN EMULSION WITH BRUSH AND WATER; MOP UP FOR DISPOSAL, AND DILUTE REMAINING SPILLED SOLVENT GREATLY WITH RUNNING WATER. IF DISPERSING AGENT NOT AVAILABLE, ADSORB ON SAND, SHOVEL INTO BUCKET(S), AND TRANSPORT TO SAFE, OPEN AREA FOR ATMOSPHERIC EVAPORATION.
    • SITE OF SPILLAGE SHOULD BE WASHED THOROUGHLY WITH WATER AND SOAP OR DETERGENT.

Ethyl alcohol (denatured)

  • COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • STORAGE: IN DARK BOTTLE ONLY.
  • SPILLAGE/DISPOSAL:
    • SHUT OFF ALL POSSIBLE SOURCES OF IGNITION
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES
    • MOP UP WITH PLENTY OF WATER
    • VENTILATE AREA WELL

Ethylenediamine

  • COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • STORAGE: IN DARK BOTTLE ONLY.
  • SPILLAGE/DISPOSAL:
    • SHUT OFF ALL POSSIBLE SOURCES OF IGNITION
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES
    • MOP UP WITH PLENTY OF WATER
    • VENTILATE AREA WELL

Isopropyl alcohol

  • COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • STORAGE: IN DARK BOTTLE ONLY.
  • SPILLAGE/DISPOSAL:
    • SHUT OFF ALL POSSIBLE SOURCES OF IGNITION
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES
    • MOP UP WITH PLENTY OF WATER
    • VENTILATE AREA WELL

Methanol

  • COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • STORAGE: IN DARK BOTTLE ONLY.
  • SPILLAGE/DISPOSAL:
    • SHUT OFF ALL POSSIBLE SOURCES OF IGNITION
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES
    • MOP UP WITH PLENTY OF WATER
    • VENTILATE AREA WELL

Propyl alcohol, normal

  • COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • STORAGE: IN DARK BOTTLE ONLY.
  • SPILLAGE/DISPOSAL:
    • SHUT OFF ALL POSSIBLE SOURCES OF IGNITION
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES
    • MOP UP WITH PLENTY OF WATER
    • VENTILATE AREA WELL

Toluene

  • COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • STORAGE: IN DARK BOTTLE ONLY.
  • SPILLAGE/DISPOSAL:
    • SHUT OFF ALL POSSIBLE SOURCES OF IGNITION
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES
    • APPLY NON-FLAMMABLE DISPERSING AGENT IF AVAILABLE AND WORK TO AN EMULSION WITH BRUSH AND WATER; MOP UP FOR DISPOSAL, AND DILUTE REMAINING SPILLED SOLVENT GREATLY WITH RUNNING WATER. IF DISPERSING AGENT NOT AVAILABLE, ADSORB ON SAND, SHOVEL INTO BUCKET(S), AND TRANSPORT TO SAFE, OPEN AREA FOR ATMOSPHERIC EVAPORATION
    • VENTILATE AREA WELL

Triethanolamine

  • SLIGHTLY COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • STORAGE: IN DARK BOTTLE ONLY.
  • SPILLAGE/DISPOSAL:
    • SHUT OFF ALL POSSIBLE SOURCES OF IGNITION.
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES, AS WELL AS PROTECTIVE CLOTHING.
    • TAKE UP WITH SAND OR OTHER NON-COMBUSTIBLE ABSORBENT MATERIAL AND PLACE INTO CONTAINER FOR LATER DISPOSAL.
    • MOP UP WITH PLENTY OF WATER.
    • VENTILATE AREA WELL.

Corrosives/irritants:

Concentrated acids will be stored in appropriate acid safety storage cabinets. Inorganic acids, as sulfuric, nitric and hydrochloric, should be stored separately from organic acids, such as acetic.

Acids and bases (including strong alkaline solutions) should not be stored in close proximity.

Since corrosive chemicals are highly reactive, dissolving and corroding (naturally) many different materials, and because some give off dangerous fumes,

  • acids and bases are to be handled in an exhaust hood or a well-ventilated area, and
  • large quantities of acids and bases should not be stored in the laboratories; instead, they will be stored in the ground-floor acid safety cabinets.

Because corrosives give off heat when diluted with water, one should always add acid to water. Doing so allows the heat to spread through the water instead of being concentrated in a small volume.

Mixing strong acids with strong bases is dangerous, and must be done slowly and with caution in a fume hood.

Since strong acids react with metals to generate explosive hydrogen gas, care much be used in dissolving metals in acids. Also, because mixing acids with solvents may lead to explosions, appropriate caution must be taken when strong oxidizers-such as nitric acid-and solvents are in proximity.

First-aid:

  • Remove any clothing saturated with spilled corrosives
  • Immediately flush affected skin areas with water for at least 15 minutes
  • Immediately flush eyes with water at an eyewash station for at least 15 minutes and seek medical attention
  • For severe exposures due to inhalation, remove victim to fresh air, keep warm and still, seek medical attention
  • For ingestion, keep victim warm and seek medical attention; DO NOT INDUCE VOMITING
  • IN ALL CASES, CHECK THE MSDS FOR THE CHEMICAL (IF KNOWN) FOR MORE SPECIFIC FIRST-AID INFORMATION

Special notes concerning corrosives/irritants used in ISAT laboratories:

Acetic acid, glacial

  • COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • SPILLAGE/DISPOSAL:
    • SHUT OFF ALL POSSIBLE SOURCES OF IGNITION.
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES, AS WELL AS PROTECTIVE CLOTHING.
    • MOP UP WITH PLENTY OF WATER AND RUN TO WASTE DILUTING GREATLY WITH RUNNING WATER.
    • VENTILATE AREA WELL.

Acetic anhydride

  • COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • SPILLAGE/DISPOSAL:
    • PERSONS NOT INVOLVED IN CLEAN-UP SHOULD BE INSTRUCTED TO KEEP A SAFE DISTANCE.
    • ADSORB ON SAND, SHOVEL INTO BUCKET(S), TRANSPORT TO SAFE PLACE AND TIP INTO LARGE VOLUME OF WATER; LEAVE TO DECOMPOSE BEFORE DECANTING WATER TO WASTE, DILUTING GREATLY WITH RUNNING WATER.
    • SITE OF SPILLAGE SHOULD BE VENTILATED AFTER WASHING THOROUGHLY WITH WATER AND SOAP OR DETERGENT.

Ammonium hydroxide; Ammonium hydroxide (28 - 30%)

  • AVOID ALL EXPOSURE TO VAPOR. USE FUME HOOD.
  • AMMONIA FUMES ARE COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES
  • SPILLAGE/DISPOSAL:
    • WEAR GOGGLES AND GLOVES (AND RUBBER BOOTS OR OVERSHOES IF SPILLAGE IS LARGE). MOP UP WITH PLENTY OF WATER AND RUN TO WASTE DILUTING GREATLY WITH RUNNING WATER.

Ethanolamine

  • AVOID ALL EXPOSURE TO VAPOR. USE FUME HOOD.
  • SPILLAGE/DISPOSAL:
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES.
    • MOP UP WITH PLENTY OF WATER AND RUN TO WASTE DILUTING GREATLY WITH RUNNING WATER.

Ethidium bromide

  • USE ONLY IN FUME HOOD, AND ONLY WITH GLOVES AND OTHER PROTECTIVE CLOTHING, TO AVOID ANY INHALATION OF THE POWDER AND SKIN CONTACT
  • SPILLAGE/DISPOSAL:
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES, AS WELL AS SELF-CONTAINED BREATHING APPARATUS AND PROTECTIVE CLOTHING.
    • USE CLEAN SHOVEL, AND PLACE MATERIAL INTO CLEAN, DRY CONTAINER, AND COVER.
    • FLUSH SPILL AREA WITH WATER, AND DISPOSE OF PROMPTLY, NOT ALLOWING IT TO ENTER DRAINS OR SEWAGE SYSTEM.

Ferric chloride

  • SPILLAGE/DISPOSAL:
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES.
    • MOP UP WITH PLENTY OF WATER AND RUN TO WASTE DILUTING GREATLY WITH RUNNING WATER.

Hydrochloric acid; Hydrochloric acid, 6 N

  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • SPILLAGE/DISPOSAL:
    • PERSONS NOT INVOLVED IN CLEAN-UP SHOULD BE INSTRUCTED TO KEEP A SAFE DISTANCE.
    • WEAR BREATHING APPARATUS, FACE SHIELD OR GOGGLES AND GLOVES, AS WELL AS PROTECTIVE CLOTHING.
    • SPREAD SODA ASH LIBERALLY OVER THE SPILLAGE AND MOP UP CAUTIOUSLY WITH WATER. RUN THIS WATER TO WASTE, DILUTING GREATLY WITH RUNNING WATER.

Nickel chloride

  • SPILLAGE/DISPOSAL:
    • THIS SOLUBLE SALT SHOULD BE DISSOLVED IN WATER AND THE SOLUTION RUN TO WASTE, DILUTING GREATLY WITH RUNNING WATER.

Phosphoric acid

  • SPILLAGE/DISPOSAL:
    • FACE SHIELD OR GOGGLES AND GLOVES, AS WELL AS PROTECTIVE CLOTHING.
    • SPREAD SODA ASH LIBERALLY OVER THE SPILLAGE AND MOP UP CAUTIOUSLY WITH WATER. RUN THIS WATER TO WASTE, DILUTING GREATLY WITH RUNNING WATER.

Pyrocatechol

  • CONSIDERED SLIGHTLY COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • SPILLAGE/DISPOSAL:
    • FACE SHIELD OR GOGGLES AND GLOVES, AS WELL AS PROTECTIVE CLOTHING.
    • MOP UP WITH PLENTY OF WATER. RUN THIS WATER TO WASTE, DILUTING GREATLY WITH RUNNING WATER.

Sulfuric acid; Sulfuric acid, 10 N

  • AVOID BREATHING VAPOR. USE FUME HOOD.
  • SPILLAGE/DISPOSAL:
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES, AS WELL AS PROTECTIVE CLOTHING.
    • SPREAD SODA ASH LIBERALLY OVER THE SPILLAGE AND MOP UP CAUTIOUSLY WITH WATER. RUN THIS WATER TO WASTE, DILUTING GREATLY WITH RUNNING WATER

Trichloroacetic acid

  • SPILLAGE/DISPOSAL:
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES, AS WELL AS PROTECTIVE CLOTHING.
    • SPREAD SODA ASH LIBERALLY OVER THE SPILLAGE AND MOP UP CAUTIOUSLY WITH WATER. RUN THIS WATER TO WASTE, DILUTING GREATLY WITH RUNNING WATER

Reproductive Toxins, High Acute Toxicity Chemicals, and Select Carcinogens:

These types of chemicals should be properly labeled with appropriate warnings and stored in well-ventilated, limited-access, areas-in unbreakable secondary containers, if possible. All containers of these substances should be properly labeled with identity and warning labels.

These chemicals should be handled, only when properly attired (especially with suitable gloves to prevent skin contact), in only a "RESTRICTED ACCESS" fume hood or area of the lab posted with special warning signs. This hood (with a face velocity of at least 60 feet per minute), or other appropriate containment device, must be used for procedures that may generate aerosols or vapors containing the substance.

The hands, forearms, face and neck of anyone who manipulates these chemicals must be washed thoroughly before that person leaves the laboratory.

If a major spill occurs outside the hood, the area involved first must be evacuated; then the cleanup personnel, wearing suitable protective apparel and equipment, should begin the cleanup process. The laboratory supervisor/instructor, as well as the Chemical Hygiene and Safety Officer, must be notified of all incidents of exposure or spills. Chemical decontamination should be used whenever possible; containers of contaminated waste (including washings from contaminated flasks) must be transferred from the controlled area in secondary containers under the supervision of the laboratory supervisor/instructor or Chemical Hygiene and Safety Officer. Contaminated waste should be stored in closed, suitably labeled secondary containers. (For liquids, the containers should be plastic bottles half-filled with vermiculite). NO CONTAMINATED WASTE MATERIALS SHOULD BE PLACED IN STANDARD LABORATORY TRASH RECEPTACLES. Contaminated clothing or shoes should be discarded or incinerated if decontamination is not possible.

The controlled work area, including any equipment, including glassware, should be decontaminated before normal work in that area is resumed.

Records of the amounts of these materials on hand, amounts used and the names of the workers involved, must be maintained by those persons overseeing laboratory activities.

At least two people must be present at all times in a laboratory where a compound in use is highly toxic or of unknown toxicity.

Breakable containers of these substances must be stored in chemically resistant trays. Work and storage surfaces are to be covered with removable, absorbent, plastic-backed paper.

If one is using toxicologically significant quantities of a select carcinogen on a regular basis (3 times per week or more), a physician should be consulted concerning desirability of regular medical surveillance.

Wet mopping-instead of dry sweeping-should be done for cleanup if the toxic substance was a dry powder.

First-aid:

  • Remove any clothing saturated with spilled solutions of poisons
  • Immediately flush affected skin areas with water for at least 15 minutes
  • Immediately flush eyes with water at an eyewash station for at least 15 minutes and seek medical attention
  • For severe exposures due to inhalation, remove victim to fresh air, keep warm and still, seek medical attention
  • For ingestion, keep victim warm and seek medical attention
  • IN ALL CASES, CHECK THE MSDS FOR THE CHEMICAL (IF KNOWN) FOR MORE SPECIFIC FIRST-AID INFORMATION

Special notes concerning toxins and carcinogens used in ISAT laboratories:

Sodium azide

  • COMBUSTIBLE. KEEP AWAY FROM OPEN FLAME AND HEAT SOURCES.
  • USE ONLY IN FUME HOOD, AND ONLY WITH GLOVES AND OTHER PROTECTIVE CLOTHING, TO AVOID ANY INHALATION OF THE POWDER AND SKIN CONTACT
  • SPILLAGE/DISPOSAL:
    • SHUT OFF ALL IGNITION SOURCES.
    • WEAR FACE SHIELD OR GOGGLES AND GLOVES, AS WELL AS SELF-CONTAINED BREATHING APPARATUS AND PROTECTIVE CLOTHING.
    • USE CLEAN SHOVEL, AND PLACE MATERIAL INTO CLEAN, DRY CONTAINER, AND COVER.
    • FLUSH SPILL AREA WITH WATER, AND DISPOSE OF PROMPTLY, NOT ALLOWING IT TO ENTER DRAINS OR SEWAGE SYSTEM.

Compressed gases, liquefied gases, and cryogenic liquids:

All gas lines leading from a compressed gas supply should be labeled clearly to identify the gas. Labels should be color-coded to distinguish hazardous gases from those that are inert.

Cylinders should be transported carefully, not dragged, rolled, slid, or allowed to strike each other forcefully. They should be transported on wheeled cylinder carts with retaining straps or chains.

Once in place, cylinders should be secured firmly, individually, by means of clamp and belt or chain, to a wall or lab bench. The valve handle at the top should be accessible at all times.

The cylinder valve should be opened slowly, only when a proper regulator is in place.

Leak-testing should be performed when a problem with a cylinder is suspected. To check for leaks, a flammable gas leak detector or soapy water, or a 50% glycerin/water solution, may be used. If a leak at the cylinder valve handle cannot be remedied by tightening a valve gland or a packing nut, emergency action should be taken and the supplier should be notified.

All sources of ignition should be kept away from cylinders of flammable gases, e.g., oxygen, hydrogen, methane and acetylene.

Cylinders should not be emptied to a pressure lower than 172 kPa (25 psi) because the residual contents may become contaminated with air if the valve is left open.

Because of the special risk of eye and skin contact of personnel who work with cryogenic liquids (e.g., liquid nitrogen, helium and argon),

  • eye protection-preferably a face shield-should be worn,
  • gloves, impervious of the fluid, must be worn, and
  • the area must be well-ventilated.


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