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Chemical-Specific Safety Procedures

Reproductive Toxins:

Reproductive toxins should be handled only in a hood, using appropriate protective apparel (especially suitable gloves) to prevent skin contact.

Reproductive toxins should be properly labeled and stored in well-ventilated areas in unbreakable secondary containers, if possible.

Notify supervisor/instructor of all incidents of exposure or spills.

High Acute Toxicity Chemicals (Supplemental rules to be followed in addition to those mentioned above):

Use and store these chemicals in areas of restricted access that are posted with special warning signs. These areas should include a hood (with a face velocity of at least 60 linear feet/minute) or other containment device for procedures that may generate aerosols or vapors containing the substance.

Use gloves, long sleeves and other protective apparel as needed to avoid skin contact. Always wash hands after working with these chemicals.

Maintain records of the amounts of these materials on hand, amounts used and the names of the workers involved.

Assure that at least two people are present at all times if a compound in use is highly toxic or of unknown toxicity.

Be prepared for accidents and spills. Store breakable containers of these substances in chemically resistant trays. Cover work and storage surfaces with removable, absorbent, plastic backed paper.

If a major spill occurs outside the hood, evacuate the area; assure that cleanup personnel wear suitable protective apparel and equipment.

Thoroughly decontaminate or incinerate contaminated clothing or shoes. If possibly, chemically decontaminate by chemical conversion.

Store contaminated waste in closed, suitably labeled, secondary containers (for liquids, plastic bottles half-filled with vermiculite).

Select Carcinogens (Further supplemental rules to be followed, in addition to all these mentioned above):

Conduct all transfers and work with these substances in a designated area--a restricted access hood, glove box or portion of a lab designated for use of highly toxic substances, for which all people with access are aware of the substance being used and necessary precautions. The designated area should be conspicuously marked with warning and restricted access signs. Prepare a plan for use and disposal of these materials and obtain approval of the appropriate lab supervisor or instructor.

All containers of these substances should be properly labeled with identity and warning labels.

Store containers of these chemicals in ventilated, limited access areas in appropriately labeled, unbreakable, chemically resistant, secondary containers.

If using toxicologically significant quantities of a select carcinogen on a regular basis (3 times per week or more), consult a physician concerning desirability of regular medical surveillance.

Use a wet mop instead of dry sweeping if the toxic substance was a dry powder.

When using a positive pressure glove box, thoroughly check for leaks before each use. Trap exit gases or filter them through a HEPA filter and then release into the hood.

Use chemical decontamination whenever possible; ensure that containers of contaminated waste (including washings from contaminated flasks) are transferred from the controlled area in secondary container under the supervision of the laboratory supervisor/instructor.

Decontaminate any equipment, including glassware, in the hood before removing them from the controlled area. Decontaminate the controlled area before resuming normal work there.

On leaving the area, remove protective apparel and wash hands, forearms, face and neck.

Flammables:

Never heat flammable liquids with an open flame or hot plate. Use a heating mantle, steam bath or hot water bath.

Never use or store flammable chemicals near any source of ignition, spark or open flame.

Handle solvents in an exhaust hood or a well-ventilated area.

Ground containers when transferring from one container to another if the potential for sparking exists.

Do not store large quantities of flammable reagents in the laboratory.

Store flammable liquids in appropriate safety cabinets and/or safety cans.

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