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James Herrick

 


 
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James Herrick

herricjb@jmu.edu
Contact Info


Associate Professor of Biology

B.S. - Brigham Young University
M.S. - Brigham Young University
Ph.D. - Cornell University

Phone - 540-568-6653
Fax - 540-568-3333
Office -  Bioscience 3028G

Office Hours

Courses:   Allied Health Microbiology (BIO 280), Microbial Ecology and Evolution (BIO 453/553), Evolution and Ecology of Infectious Disease (BIO 447/547)


Research Interests:  Environmental and Molecular Microbiology

In our laboratory, we use molecular, genetic, and microbiological techniques to study populations of native and introduced -- including pathogenic -- bacteria in natural streams and soils. Our main interest is in the lateral transfer of genes to and among bacteria in streams and soils. Lateral gene transfer - as opposed to the 'vertical' transfer of genes via simple cell division - allows genes to move between mature cells and thus to potentially spread very quickly through a population and even from species to species. Lateral gene transfer has had a profound effect on genome evolution, on pollutant biodegradation, and particularly on the development of antibiotic resistance in bacteria. Our current focus is on the transfer of resistance genes in stream waters and sediments. Antibiotic overuse and misuse may lead to selection for resistance genes, many of which are found on mobile genetic elements such as plasmids and transposons, and these can potentially be transferred from human or animal strains to bacteria in the environment. Resistant environmental bacterial populations may then act as environmental reservoirs and evolutionary "incubators" of resistance genes, thus providing new variants and combinations of resistance phenotypes for subsequent transfer to human and animal pathogenic bacteria. We are currently using genetic 'capture' techniques and comparative genomics to study actively transferring resistance plasmids which, though acquired from native stream bacteria, may have their origins in introduced, antibiotic-selected fecal bacteria.

In a recent collaboration with the laboratory of Dr. Reid Harris, we have been studying the bacteria inhabiting the skin of amphibians, with particular attention to those able to inhibit the deleterious effects of the chytrid fungus devastating many amphibious populations worldwide. We are currently using metagenomic approaches to investigate the microbiome of the Eastern red-backed salamander. We are also investivating the occurrence of Staphylococcus in stream sediments.


Selected Publications:

Herrick, J.B., Haynes, R., Heringa, S., Brooks, J., and Sobota, L. 2014. Co-selection for resistance to multiple late-generation human therapeutic antibiotics encoded on tetracycline resistance plasmids captured from uncultivated stream and soil bacteria. Journal of Applied Microbiology (in press)

Muletz, C.R., Myers, J.M., Domangue, R.J., Herrick, J.B. and Harris, R.N. 2012. Soil bioaugmentation with amphibian cutaneous bacteria protects amphibian hosts from infection by Batrachochytrium dendrobatidis. Biological Conservation, 152, 119-126.

Heringa, S. D., J. Monroe, and J. Herrick. 2007. A simple, rapid method for extracting large plasmid DNA from bacteria. Nature Precedings <http://dx.doi.org/10.1038/npre.2007.1249.1>

Wilson, M.S., J. B. Herrick, C. O. Jeon, D. E. Hinman, and E. L. Madsen. 2003. Horizontal transfer of phn-Ac dioxygenase genes within one of two phenotypically and genotypically distinctive naphthalene-degrading guilds from adjacent soil environments. Appl. Environ. Microbiol. 69: 2172-2181.

Stuart-Keil, K.G., A. M. Hohnstock, K. P. Drees, J. B. Herrick, and E. L. Madsen. 1998. Plasmids responsible for horizontal transfer of naphthalene catabolism genes between bacteria at a coal tar-contaminated site are homologous to pDTG1 from Pseudomonas putida NCIB 9816-4. Appl. Environ. Microbiol. 64: 3633-3640.

Herrick, J. B., E. L. Madsen, and W. C. Ghiorse. 1997. Natural horizontal transfer of a naphthalene dioxygenase gene between bacteria native to a coal tar-contaminated field site. Appl. Environ. Microbiol. 63: 2330-2337.

Herrick, J. B., D. N. Miller, E. L. Madsen, and W. C. Ghiorse. 1996. Extraction, purification, and PCR amplification of microbial DNA from sediments and soils. In PCR: Essential Techniques, Julian F. Burke (ed.). Bios Sci. Publ., Oxford.

Ghiorse, W. C., J. B. Herrick, R. L. Sandoli, and E. L. Madsen. 1995. Natural selection of PAH-degrading bacterial guilds at coal tar disposal sites. Environmental Health Perspectives 103 (Suppl. 5): 107-111.

More', M. I., J. B. Herrick, M. C. Silva, W. C. Ghiorse, and E. L. Madsen. 1994. Quantitative cell lysis of indigenous microorganisms and rapid extraction of microbial DNA from sediment. Appl. Environ. Microbiol. 60 (5): 1572-1580.

Herrick, J. B., E. L. Madsen, C. A. Batt, and W. C. Ghiorse. 1993. Polymerase chain reaction amplification of naphthalene catabolic and 16S rRNA gene sequences from indigenous sediment bacteria. Appl. Environ. Microbiol. 59 (3): 687-694.